![]() It is believed that fibers formed by electrospinning have the ability to mimic, in a limited way, the natural extracellular matrix structure regarding variability of fiber diameter, topology, texture, and mechanical properties (16). The mechanical properties of the scaffolds provide enough space to accommodate cells and an easy passage for nutrient intake and metabolic waste exchange (14). Scaffolds produced by this method are porous and have a large surface-to-volume ratio. Many parameters are involved in the failure of the final fiber, such as the applied voltage, tip-target distance, feeding rate, concentration and viscosity of the polymer solution, and temperature, among others (14,15). This technique is employed to produce nanofibers with a diameter in the range of nanometers to micrometers by the use of electrostatic forces. This approach has a great potential for many tissues such as vessels (4,5), bone (6,7), neural tissue (8,9), cartilage (10,11), and tendons/ligaments (12,13).Īmong the various types of materials used in tissue engineering, scaffolds based on nanofibers produced by electrospinning have been widely studied. One of the most important goals in this area is to associate cells with biomaterials to provide a scaffold on which they can anchor, migrate and proliferate three-dimensionally. Tissue engineering is a growing field with the potential to regenerate damaged tissue (1-3). Key words: Nanotechnology Tissue engineering Electrospinning Stem cells Netto 2 andġLaboratório de Hematologia e Células-tronco, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrasilĢLaboratório de Isquemia Cerebral, Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrasilģHospital Nossa Senhora da Conceição, Grupo Hospitalar Conceição, Porto Alegre, RS, BrasilĤInstituto de Pesquisa com Células-tronco, Porto Alegre, RS, Brasil Viability of mesenchymal stem cells during electrospinning Nanotechnology Tissue engineering Electrospinning Stem cellsĪbstract Introduction Material and Methods Results Discussion References Acknowledgmentsīraz J Med Biol Res, February 2012, Volume 45(2) 125-130 These results suggest that the incorporation of cells during fiber formation by electrospinning is a viable process that needs more investigation in order to find ways to protect cells from damage. The loss of viability was possibly due to the high viscosity of the polymer solution, which reduced the access to nutrients associated with electric and mechanical stress during electrospinning. After electrospinning, the viability of mesenchymal stem cells was reduced from 88 to 19.6% and the viability of mononuclear cells from 99 to 8.38%. Fiber diameter was observed by scanning electron microscopy and the presence of cells within the scaffolds was analyzed by confocal laser scanning microscopy. Cell viability was assessed before and after the procedure by exclusion of dead cells using trypan blue staining. Cells were re-suspended in a 10% polyvinyl alcohol solution and subjected to electrospinning for 30 min under a voltage of 21 kV. Mesenchymal stem cells were extracted from the wall of the umbilical cord and mononuclear cells from umbilical cord blood. In the present study, a different approach for cell incorporation into fibrous scaffolds was tested. Electrospinning is a technique that facilitates the production of nanofibers and is commonly used to develop fibrous scaffolds to be used in tissue engineering. Tissue engineering is a technique by which a live tissue can be re-constructed and one of its main goals is to associate cells with biomaterials. ![]() , Instituto de Pesquisa com Células-tronco, Brasil About the authors , Universidade Federal do Rio Grande do Sul, Departamento de Bioquímica, Laboratório de Isquemia Cerebral, Brazil P. , Grupo Hospitalar Conceição, Hospital Nossa Senhora da Conceição, Brazil C.A. , Universidade Federal do Rio Grande do Sul, Faculdade de Farmácia, Laboratório de Hematologia e Células-tronco, Brazil R.A. , Universidade Federal do Rio Grande do Sul, Faculdade de Farmácia, Laboratório de Hematologia e Células-tronco, Brazil D.I. , Universidade Federal do Rio Grande do Sul, Departamento de Bioquímica, Laboratório de Isquemia Cerebral, Brazil D. , Universidade Federal do Rio Grande do Sul, Faculdade de Farmácia, Laboratório de Hematologia e Células-tronco, Brazil ![]()
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